HSC3-CAS9 cell line

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HSC3-CAS9 Order

Catalog#

YC-C063-Cas9-H

Size

1*10^6

Transfection Culture Medium

Transfection Culture medium-A

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

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Detailed Product Information

Product category

Cas9 Stable Cell Lines

cell name

HSC3-CAS9

Cell Morphology

Endothelial cells, adherent

Passage ratio

1:3~1:5

Species

Human

Expression gene

Cas9

Resistance gene

hygro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10%FBS

Description

Use Cas9 gene expressing lentivirus constructed by Ubigene to infect the HSC3 cell line， screen the cells based on the resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a HSC3-CAS9 cell line that can maintain long-term, stable and high expression.

Product Validation Data（RT-QPCR）

Sample Name	Target Name	Cт Mean	ΔCт
HSC3-CAS9	Cas9	18.04600000	1.18300000
HSC3-CAS9	β-actin	16.86300000	1.18300000
HSC3	Cas9	27.67400000	8.93800000
HSC3	β-actin	18.73600000	8.93800000

Cutting Efficiency Validation

HSC3-CAS9 cell line Product Validation Data（RT-QPCR）

Note: The above figure shows the sequencing peaks of the HSC3-CAS9 stable cell pool which is electroporated by IGF2BP2 gene targeting gRNA plasmid, after 48h antibiotic screening. The red arrow indicates the position where the nested peak appears, which shows that the genotype of the target site is significantly changed due to the cutting. Therefore, it indicates that Cas9 nuclease is successfully expressed.

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:HSC3			STR Info (Cell bank) Cell Line:HSC-3
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	13		11	13
D13S317	12	12		12	12
D7S820	13	13		13	13
D16S539	9	9		9	9
VWA	14	17		14	17
TH01	6	9.3		6	9.3
AMEL	X	Y		X	Y
TPOX	8	8		8	8
CSF1PO	11	11		11	11
D12S391	18	18
FGA	22	26
D2S1338	19	23
D21S11	30	31.2
D18S51	15	15
D8S1179	12	12
D3S1358	17	17
D6S1043	13	19
PENTAE	11	15
D19S433	14.2	14.2
PENTAD	10	10
D1S1656	13	16

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Product Advantage

Easy to use：Cas9 cell lines in our cell bank can stably express Cas9 protein. Each Cas9 Stable Cell Line is easy to use and enables gene knockout simply by transfecting gRNA, while transfection of gRNA and donor DNA results in gene knock-in or point mutations
01
High-efficient KO：These Cas9 stable cell lines have been used to construct the KO cell lines for various genes, gene KO efficiency 5-10 times improved.
02
High-quality cell：Selected cells, low passages, good cell condition, high activity, applicable for all kinds of gene-editing experiments.
03

HSC3-CAS9

Catalog#

YC-C063-Cas9-H

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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HSC3-CAS9 cell line

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